Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2557362..2557859 Replicon chromosome
Accession NZ_CP074028
Organism Escherichia coli strain PK13

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KFU76_RS12460 Protein ID WP_001173343.1
Coordinates 2557362..2557535 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KFU76_RS12465 Protein ID WP_000398619.1
Coordinates 2557563..2557859 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU76_RS12450 (2554032) 2554032..2555489 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
KFU76_RS12455 (2555726) 2555726..2557243 + 1518 WP_001295074.1 lysine--tRNA ligase -
KFU76_RS12460 (2557362) 2557362..2557535 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KFU76_RS12465 (2557563) 2557563..2557859 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KFU76_RS12470 (2558086) 2558086..2558358 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
KFU76_RS12475 (2558370) 2558370..2558600 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
KFU76_RS12480 (2558781) 2558781..2560412 + 1632 WP_001216461.1 sensor histidine kinase -
KFU76_RS12485 (2560409) 2560409..2561128 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T201176 WP_001173343.1 NZ_CP074028:c2557535-2557362 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T201176 NZ_CP098229:1889845-1890096 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT201176 WP_000398619.1 NZ_CP074028:c2557859-2557563 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT201176 NZ_CP098229:1890093-1890347 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References