Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 2454867..2455462 Replicon chromosome
Accession NZ_CP074028
Organism Escherichia coli strain PK13

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag KFU76_RS11945 Protein ID WP_000239579.1
Coordinates 2454867..2455217 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag KFU76_RS11950 Protein ID WP_001223208.1
Coordinates 2455211..2455462 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU76_RS11925 (2450143) 2450143..2451165 - 1023 WP_001339490.1 ABC transporter permease -
KFU76_RS11930 (2451179) 2451179..2452681 - 1503 WP_000205793.1 sugar ABC transporter ATP-binding protein -
KFU76_RS11935 (2452991) 2452991..2453947 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
KFU76_RS11940 (2454257) 2454257..2454787 + 531 WP_000055072.1 inorganic diphosphatase -
KFU76_RS11945 (2454867) 2454867..2455217 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
KFU76_RS11950 (2455211) 2455211..2455462 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
KFU76_RS11955 (2455675) 2455675..2456016 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
KFU76_RS11960 (2456019) 2456019..2459798 - 3780 WP_000060901.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T201175 WP_000239579.1 NZ_CP074028:c2455217-2454867 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T201175 NZ_CP098229:1382829-1383227 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT201175 WP_001223208.1 NZ_CP074028:c2455462-2455211 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT201175 NZ_CP098229:1382602-1382829 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References