Detailed information of TA system TA201173

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1829943..1830780
• Replicon: chromosome
• Accession: NZ_CP074028
• Organism: Escherichia coli strain PK13

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: KFU76_RS09030
• Protein ID: WP_000227784.1
• Coordinates: 1830238..1830780 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: KFU76_RS09025
• Protein ID: WP_001297137.1
• Coordinates: 1829943..1830254 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU76_RS09000 (1824963)	1824963..1825910	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
KFU76_RS09005 (1825932)	1825932..1827923	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
KFU76_RS09010 (1827913)	1827913..1828527	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
KFU76_RS09015 (1828527)	1828527..1828856	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
KFU76_RS09020 (1828868)	1828868..1829758	+	891	WP_000971336.1	heme o synthase	-
KFU76_RS09025 (1829943)	1829943..1830254	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
KFU76_RS09030 (1830238)	1830238..1830780	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
KFU76_RS09035 (1830836)	1830836..1831771	-	936	WP_106661391.1	tetratricopeptide repeat protein	-
KFU76_RS09040 (1832179)	1832179..1833543	+	1365	WP_001000975.1	MFS transporter	-
KFU76_RS09045 (1833671)	1833671..1834162	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
KFU76_RS09050 (1834330)	1834330..1835241	+	912	WP_000705847.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T201173 WP_000227784.1 NZ_CP074028:1830238-1830780 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T201173 NZ_CP098229:924907-925314 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT201173 WP_001297137.1 NZ_CP074028:1829943-1830254 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT201173 NZ_CP098229:924660-924926 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3