Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1795866..1796484 Replicon chromosome
Accession NZ_CP074028
Organism Escherichia coli strain PK13

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KFU76_RS08860 Protein ID WP_001291435.1
Coordinates 1796266..1796484 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KFU76_RS08855 Protein ID WP_000344800.1
Coordinates 1795866..1796240 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU76_RS08845 (1790955) 1790955..1792148 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KFU76_RS08850 (1792171) 1792171..1795320 + 3150 WP_257171086.1 efflux RND transporter permease AcrB -
KFU76_RS08855 (1795866) 1795866..1796240 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KFU76_RS08860 (1796266) 1796266..1796484 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KFU76_RS08865 (1796656) 1796656..1797207 + 552 WP_000102568.1 maltose O-acetyltransferase -
KFU76_RS08870 (1797323) 1797323..1797793 + 471 WP_000136192.1 YlaC family protein -
KFU76_RS08875 (1797957) 1797957..1799507 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KFU76_RS08880 (1799549) 1799549..1799902 - 354 WP_000878140.1 DUF1428 domain-containing protein -
KFU76_RS08890 (1800281) 1800281..1800592 + 312 WP_000409911.1 MGMT family protein -
KFU76_RS08895 (1800623) 1800623..1801195 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T201172 WP_001291435.1 NZ_CP074028:1796266-1796484 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T201172 NZ_CP098229:c826228-825854 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGAAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTGTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT201172 WP_000344800.1 NZ_CP074028:1795866-1796240 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT201172 NZ_CP098229:c826686-826318 [Escherichia coli]
GTGTCAGACACATTCTCCGGGACAACTCATCCCGATGACAATCACGACCGTCCCTGGTGGGGACTACCCAGCACCGTGAC
GCCCTGTTTCGGGGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGAACCATCTGGACCAAGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGTTCACCAGCAGT
GAACTGAATCCCCACCGTCAGAATACCGTCACGCTGTATGCTAAAGGGCTGACCTGCCATGCGGACACCCTCGGCAGCTG
TGGTTACGTTTATCTTGCTGTTTATCCGACGCCCGAAACGAAACAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References