Detailed information of TA system TA201169

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 579514..580040
• Replicon: chromosome
• Accession: NZ_CP074028
• Organism: Escherichia coli strain PK13

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: KFU76_RS02950
• Protein ID: WP_000323025.1
• Coordinates: 579753..580040 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: KFU76_RS02945
• Protein ID: WP_000534858.1
• Coordinates: 579514..579753 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU76_RS02915 (574852)	574852..575341	+	490	Protein_571	class I SAM-dependent methyltransferase	-
KFU76_RS02920 (575872)	575872..576104	+	233	Protein_572	hypothetical protein	-
KFU76_RS02925 (576893)	576893..577942	+	1050	Protein_573	ISNCY family transposase	-
KFU76_RS02930 (578063)	578063..578212	+	150	WP_011443592.1	protein YdfW	-
KFU76_RS02935 (578649)	578649..578981	-	333	WP_001301033.1	FlxA-like family protein	-
KFU76_RS02940 (579184)	579184..579489	-	306	WP_071593935.1	protein YdfV	-
KFU76_RS02945 (579514)	579514..579753	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
KFU76_RS02950 (579753)	579753..580040	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
KFU76_RS02955 (580112)	580112..580267	+	156	WP_001406737.1	type I toxin-antitoxin system toxin HokD	-
KFU76_RS02960 (580558)	580558..581720	+	1163	WP_085947771.1	IS3-like element IS3 family transposase	-
KFU76_RS02965 (581755)	581755..581997	+	243	WP_106661496.1	protein Rem	-
KFU76_RS02970 (582064)	582064..582342	+	279	WP_001515373.1	hypothetical protein	-
KFU76_RS02975 (582344)	582344..583393	+	1050	WP_106661497.1	DUF968 domain-containing protein	-
KFU76_RS02980 (583411)	583411..583788	+	378	WP_001204787.1	antiterminator Q family protein	-
KFU76_RS02985 (583944)	583944..584468	-	525	WP_000780584.1	lipocalin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	566375..629250	62875

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T201169 WP_000323025.1 NZ_CP074028:579753-580040 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T201169 NZ_CP098229:298722-298952 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT201169 WP_000534858.1 NZ_CP074028:579514-579753 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT201169 NZ_CP098229:298949-299308 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8