Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2483295..2483933 Replicon chromosome
Accession NZ_CP074024
Organism Escherichia coli strain PK5

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag KFU66_RS12125 Protein ID WP_000813794.1
Coordinates 2483757..2483933 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag KFU66_RS12120 Protein ID WP_001270286.1
Coordinates 2483295..2483711 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU66_RS12100 (2478448) 2478448..2479388 - 941 Protein_2369 ABC transporter permease -
KFU66_RS12105 (2479389) 2479389..2480402 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
KFU66_RS12110 (2480420) 2480420..2481565 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
KFU66_RS12115 (2481810) 2481810..2483216 - 1407 WP_219371243.1 PLP-dependent aminotransferase family protein -
KFU66_RS12120 (2483295) 2483295..2483711 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
KFU66_RS12125 (2483757) 2483757..2483933 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
KFU66_RS12130 (2484155) 2484155..2484385 + 231 WP_000494244.1 YncJ family protein -
KFU66_RS12135 (2484477) 2484477..2486438 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
KFU66_RS12140 (2486511) 2486511..2487047 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
KFU66_RS12145 (2487139) 2487139..2488314 + 1176 WP_257188421.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T201153 WP_000813794.1 NZ_CP074024:c2483933-2483757 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T201153 NZ_CP098227:2029364-2029467 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT201153 WP_001270286.1 NZ_CP074024:c2483711-2483295 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT201153 NZ_CP098227:c2029505-2029234 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References