Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2378574..2380162 Replicon chromosome
Accession NZ_CP074024
Organism Escherichia coli strain PK5

Toxin (Protein)


Gene name hipA Uniprot ID S1ELM3
Locus tag KFU66_RS11690 Protein ID WP_001125439.1
Coordinates 2378840..2380162 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID -
Locus tag KFU66_RS11685 Protein ID WP_001301023.1
Coordinates 2378574..2378840 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU66_RS11685 (2378574) 2378574..2378840 + 267 WP_001301023.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
KFU66_RS11690 (2378840) 2378840..2380162 + 1323 WP_001125439.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
KFU66_RS11695 (2380361) 2380361..2380732 - 372 WP_055313351.1 hypothetical protein -
KFU66_RS11700 (2380775) 2380775..2381242 - 468 WP_048242157.1 hypothetical protein -
KFU66_RS11705 (2381242) 2381242..2381511 - 270 WP_001610304.1 hypothetical protein -
KFU66_RS11710 (2381743) 2381743..2383140 - 1398 WP_257188418.1 ISNCY family transposase -
KFU66_RS11715 (2383432) 2383432..2384832 + 1401 WP_072142668.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2381743..2383140 1397


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(149-401)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49275.82 Da        Isoelectric Point: 8.2482

>T201152 WP_001125439.1 NZ_CP074024:2378840-2380162 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Download         Length: 1323 bp

>T201152 NZ_CP098227:1023621-1023956 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGCTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10016.38 Da        Isoelectric Point: 6.2267

>AT201152 WP_001301023.1 NZ_CP074024:2378574-2378840 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Download         Length: 267 bp

>AT201152 NZ_CP098227:1023373-1023621 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5K98
PDB 3TPV
PDB 3DNV
PDB 3TPD
PDB 3HZI
PDB 3FBR
PDB 4YG7
PDB 3TPE
PDB 2WIU
PDB 3DNT
PDB 3TPB
PDB 3TPT
PDB 3DNU

Antitoxin

Source ID Structure

References