Detailed information of TA system TA020115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): VapXD/-
• Location: 473074..473549
• Replicon: chromosome
• Accession: NC_000907
• Organism: Haemophilus influenzae Rd KW20

Toxin (Protein)

• Gene name: VapD
• Uniprot ID: -
• Locus tag: HI_RS02350
• Protein ID: WP_005693712.1
• Coordinates: 473074..473349 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: VapX
• Uniprot ID: A0A0M1GIA6
• Locus tag: HI_RS02355
• Protein ID: WP_005649350.1
• Coordinates: 473358..473549 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI_RS02330 (HI0446)	468265..469935	-	1671	WP_005693715.1	fructose-specific PTS transporter subunit EIIC	-
HI_RS02335 (HI0447)	469937..470878	-	942	WP_005693714.1	1-phosphofructokinase	-
HI_RS02340 (HI0448)	470880..472379	-	1500	WP_005693713.1	fused PTS fructose transporter subunit IIA/HPr protein	-
HI_RS02345 (HI0449)	472451..472981	-	531	WP_005686915.1	hypothetical protein	-
HI_RS02350 (HI0450)	473074..473349	-	276	WP_005693712.1	virulence-associated protein VapD	Toxin
HI_RS02355 (HI0451)	473358..473549	-	192	WP_005649350.1	DUF5397 domain-containing protein	Antitoxin
HI_RS02360 (HI0452)	473621..474919	-	1299	WP_010868987.1	hemolysin family protein	-
HI_RS02365 (HI0453)	474970..475494	-	525	WP_005652096.1	DUF1523 family protein	-
HI_RS02370 (HI0454)	475521..476303	-	783	WP_010868988.1	YchF/TatD family DNA exonuclease	-
HI_RS02375 (HI0455)	476358..477341	-	984	WP_010868989.1	DNA polymerase III subunit delta'	-
HI_RS02380 (HI0456)	477338..477970	-	633	WP_032828392.1	dTMP kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Virulence-associated protein D / CRISPR associated protein Cas2 (6-85)

Antitoxin

• Protein of unknown function DUF5397 (1-63)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10543.06 Da
• Isoelectric Point: 7.0053

>T20115 WP_005693712.1 NC_000907:c473349-473074 [Haemophilus influenzae Rd KW20]
MYAIAFLVVKDTQDYHPKGVQQAYTDIGAVLAKFGFVRTQGSLYINMNEDMANLFQAMNALKQLAWISQSVRDIRAFRIE
QWSDFTDFIRN

Nucleotide

• Length: 276 bp

>T20115 NC_000907:c473349-473074 [Haemophilus influenzae Rd KW20]
ATGTACGCGATTGCTTTTTTAGTTGTAAAAGACACTCAAGATTATCATCCAAAGGGCGTTCAACAGGCTTATACAGACAT
TGGCGCAGTCTTAGCAAAATTTGGTTTTGTTAGAACACAAGGAAGTTTATATATCAACATGAATGAAGATATGGCGAATC
TCTTTCAGGCAATGAATGCACTAAAACAGTTGGCGTGGATTTCTCAGTCGGTACGAGATATTCGTGCTTTTCGTATTGAG
CAATGGTCTGATTTTACTGATTTTATACGAAATTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7047.02 Da
• Isoelectric Point: 3.9930

>AT20115 WP_005649350.1 NC_000907:c473549-473358 [Haemophilus influenzae Rd KW20]
MELRQQIPTGCIKQFGQFGVPYVVGEVAEFLPDGDVLVNITLLQSGEKDIYRLSHLLEDPEAE

Nucleotide

• Length: 192 bp

>AT20115 NC_000907:c473549-473358 [Haemophilus influenzae Rd KW20]
ATGGAACTAAGACAACAAATTCCAACAGGCTGTATTAAGCAATTCGGGCAATTTGGCGTGCCTTATGTGGTTGGCGAGGT
TGCGGAATTTTTGCCAGATGGTGATGTGTTGGTGAATATCACATTGTTACAATCTGGTGAAAAAGACATTTATCGCTTAT
CTCACCTGCTTGAAGATCCCGAAGCTGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0M1GIA6