Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 866498..867152 Replicon chromosome
Accession NZ_CP074024
Organism Escherichia coli strain PK5

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KFU66_RS04255 Protein ID WP_000244777.1
Coordinates 866745..867152 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KFU66_RS04250 Protein ID WP_000354046.1
Coordinates 866498..866764 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU66_RS04225 (861667) 861667..862410 + 744 WP_000951964.1 SDR family oxidoreductase -
KFU66_RS04230 (862467) 862467..863900 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
KFU66_RS04235 (863945) 863945..864256 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
KFU66_RS04240 (864420) 864420..865079 + 660 WP_000250274.1 hemolysin III family protein -
KFU66_RS04245 (865275) 865275..866255 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KFU66_RS04250 (866498) 866498..866764 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KFU66_RS04255 (866745) 866745..867152 + 408 WP_000244777.1 protein YgfX Toxin
KFU66_RS04260 (867192) 867192..867713 - 522 WP_001055874.1 flavodoxin FldB -
KFU66_RS04265 (867825) 867825..868721 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KFU66_RS04270 (868746) 868746..869456 + 711 WP_219371038.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KFU66_RS04275 (869462) 869462..871195 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T201141 WP_000244777.1 NZ_CP074024:866745-867152 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T201141 NZ_CP098223:c4562552-4562445 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGTCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTCGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT201141 WP_000354046.1 NZ_CP074024:866498-866764 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT201141 NZ_CP098223:4562609-4562667 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References