Detailed information of TA system TA201130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 65479..66104
• Replicon: plasmid pYLPM22a
• Accession: NZ_CP074020
• Organism: Escherichia coli strain PM22

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KFU87_RS23385
• Protein ID: WP_000911313.1
• Coordinates: 65706..66104 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: KFU87_RS23380
• Protein ID: WP_000450520.1
• Coordinates: 65479..65706 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU87_RS23380 (65479)	65479..65706	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
KFU87_RS23385 (65706)	65706..66104	+	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
KFU87_RS23390 (66113)	66113..68266	-	2154	WP_000009324.1	type IV conjugative transfer system coupling protein TraD	-
KFU87_RS23395 (68519)	68519..69229	-	711	WP_230133085.1	conjugal transfer complement resistance protein TraT	-
KFU87_RS23400 (69282)	69282..69779	-	498	WP_000605857.1	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55 / tet(A) / aph(3')-Ia / ant(3'')-Ia / lnu(F) / aac(3)-IIa / sitABCD	iutA / iucD / iucC / iucB / iucA	1..120320	120320

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T201130 WP_000911313.1 NZ_CP074020:65706-66104 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T201130 NZ_CP098223:816435-816749 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTGCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTAATCCATTAACACCTATTGAACGATTTAGCCATATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACACGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACTTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT201130 WP_000450520.1 NZ_CP074020:65479-65706 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT201130 NZ_CP098223:816199-816432 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAATG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAACTTCTAG

Structures

Antitoxin

• AlphaFold DB: V0VCB2