Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 21105..21748 Replicon plasmid pYLPM22a
Accession NZ_CP074020
Organism Escherichia coli strain PM22

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag KFU87_RS23130 Protein ID WP_001044768.1
Coordinates 21332..21748 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag KFU87_RS23125 Protein ID WP_001261287.1
Coordinates 21105..21335 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU87_RS23105 (16265) 16265..17353 + 1089 WP_000952217.1 transcriptional repressor PifC -
KFU87_RS23110 (17355) 17355..19580 + 2226 WP_000698737.1 P-loop NTPase fold protein -
KFU87_RS23115 (19630) 19630..20529 - 900 WP_000963206.1 nucleotide-binding protein -
KFU87_RS23120 (20519) 20519..20809 - 291 WP_000111771.1 hypothetical protein -
KFU87_RS23125 (21105) 21105..21335 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KFU87_RS23130 (21332) 21332..21748 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
KFU87_RS23135 (21910) 21910..24048 - 2139 WP_000350638.1 AAA family ATPase -
KFU87_RS23140 (24402) 24402..24659 + 258 WP_000343085.1 hypothetical protein -
KFU87_RS23145 (24659) 24659..25249 + 591 WP_000194575.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-55 / tet(A) / aph(3')-Ia / ant(3'')-Ia / lnu(F) / aac(3)-IIa / sitABCD iutA / iucD / iucC / iucB / iucA 1..120320 120320


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T201125 WP_001044768.1 NZ_CP074020:21332-21748 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T201125 NZ_CP098220:c102159-101779 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT201125 WP_001261287.1 NZ_CP074020:21105-21335 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT201125 NZ_CP098220:c102380-102159 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References