Detailed information of TA system TA201123

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4288901..4289532
• Replicon: chromosome
• Accession: NZ_CP074019
• Organism: Escherichia coli strain PM22

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: F4NNK6
• Locus tag: KFU87_RS20655
• Protein ID: WP_001259388.1
• Coordinates: 4289257..4289532 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A8S7TCM0
• Locus tag: KFU87_RS20650
• Protein ID: WP_000593556.1
• Coordinates: 4288901..4289260 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU87_RS20625 (4285033)	4285033..4285977	+	945	WP_000947068.1	nickel ABC transporter permease subunit NikB	-
KFU87_RS20630 (4285974)	4285974..4286807	+	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
KFU87_RS20635 (4286807)	4286807..4287571	+	765	WP_001136236.1	nickel import ATP-binding protein NikD	-
KFU87_RS20640 (4287568)	4287568..4288374	+	807	WP_000173666.1	nickel import ATP-binding protein NikE	-
KFU87_RS20645 (4288380)	4288380..4288781	+	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
KFU87_RS20650 (4288901)	4288901..4289260	-	360	WP_000593556.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
KFU87_RS20655 (4289257)	4289257..4289532	-	276	WP_001259388.1	type II toxin-antitoxin system HicA family toxin	Toxin
KFU87_RS20660 (4289605)	4289605..4290729	-	1125	WP_001314210.1	ABC transporter permease	-
KFU87_RS20665 (4290729)	4290729..4293464	-	2736	WP_000149125.1	ribosome-associated ATPase/putative transporter RbbA	-
KFU87_RS20670 (4293461)	4293461..4294528	-	1068	WP_000361485.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10151.89 Da
• Isoelectric Point: 10.7076

>T201123 WP_001259388.1 NZ_CP074019:c4289532-4289257 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T201123 NZ_CP098220:4487-4792 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13388.25 Da
• Isoelectric Point: 5.1987

>AT201123 WP_000593556.1 NZ_CP074019:c4289260-4288901 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHV

Nucleotide

• Length: 360 bp

>AT201123 NZ_CP098220:4267-4485 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FFA2