Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3709244..3709898 Replicon chromosome
Accession NZ_CP074019
Organism Escherichia coli strain PM22

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag KFU87_RS17720 Protein ID WP_000244772.1
Coordinates 3709244..3709651 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KFU87_RS17725 Protein ID WP_000354046.1
Coordinates 3709632..3709898 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU87_RS17700 (3705201) 3705201..3706934 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
KFU87_RS17705 (3706940) 3706940..3707650 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KFU87_RS17710 (3707675) 3707675..3708571 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KFU87_RS17715 (3708683) 3708683..3709204 + 522 WP_001055874.1 flavodoxin FldB -
KFU87_RS17720 (3709244) 3709244..3709651 - 408 WP_000244772.1 protein YgfX Toxin
KFU87_RS17725 (3709632) 3709632..3709898 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KFU87_RS17730 (3710141) 3710141..3711121 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KFU87_RS17735 (3711198) 3711198..3711857 - 660 WP_000250274.1 hemolysin III family protein -
KFU87_RS17740 (3712021) 3712021..3712332 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
KFU87_RS17745 (3712377) 3712377..3713810 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
KFU87_RS17750 (3713867) 3713867..3714610 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T201121 WP_000244772.1 NZ_CP074019:c3709651-3709244 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T201121 NZ_CP098219:c4678475-4678368 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGTTCCGGTCATTACTGGCATTCTTGCCAGTATGAT
CGTGAGCTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT201121 WP_000354046.1 NZ_CP074019:c3709898-3709632 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT201121 NZ_CP098219:4678532-4678589 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References