Detailed information of TA system TA201119

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3246570..3247195
• Replicon: chromosome
• Accession: NZ_CP074019
• Organism: Escherichia coli strain PM22

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A8S7SZU1
• Locus tag: KFU87_RS15550
• Protein ID: WP_000911327.1
• Coordinates: 3246570..3246968 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: KFU87_RS15555
• Protein ID: WP_000450524.1
• Coordinates: 3246968..3247195 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU87_RS15530 (3242448)	3242448..3242648	+	201	WP_000383836.1	YpfN family protein	-
KFU87_RS15535 (3242758)	3242758..3243456	-	699	WP_000679823.1	esterase	-
KFU87_RS15540 (3243530)	3243530..3245545	-	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
KFU87_RS15545 (3245560)	3245560..3246423	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
KFU87_RS15550 (3246570)	3246570..3246968	-	399	WP_000911327.1	type II toxin-antitoxin system VapC family toxin	Toxin
KFU87_RS15555 (3246968)	3246968..3247195	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
KFU87_RS15560 (3247350)	3247350..3248063	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
KFU87_RS15565 (3248276)	3248276..3249310	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
KFU87_RS15570 (3249327)	3249327..3250205	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
KFU87_RS15575 (3250351)	3250351..3250923	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
KFU87_RS15580 (3250923)	3250923..3251393	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T201119 WP_000911327.1 NZ_CP074019:c3246968-3246570 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMEIIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T201119 NZ_CP098219:4265330-4265794 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT201119 WP_000450524.1 NZ_CP074019:c3247195-3246968 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT201119 NZ_CP098219:4264995-4265330 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33