Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1086592..1087210 Replicon chromosome
Accession NZ_CP074019
Organism Escherichia coli strain PM22

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KFU87_RS05120 Protein ID WP_001291435.1
Coordinates 1086592..1086810 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KFU87_RS05125 Protein ID WP_000344800.1
Coordinates 1086836..1087210 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU87_RS05085 (1081881) 1081881..1082453 + 573 WP_023156216.1 YbaY family lipoprotein -
KFU87_RS05090 (1082484) 1082484..1082795 - 312 WP_000409911.1 MGMT family protein -
KFU87_RS05100 (1083174) 1083174..1083527 + 354 WP_000878140.1 DUF1428 domain-containing protein -
KFU87_RS05105 (1083569) 1083569..1085119 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KFU87_RS05110 (1085283) 1085283..1085753 - 471 WP_000136192.1 YlaC family protein -
KFU87_RS05115 (1085869) 1085869..1086420 - 552 WP_000102564.1 maltose O-acetyltransferase -
KFU87_RS05120 (1086592) 1086592..1086810 - 219 WP_001291435.1 HHA domain-containing protein Toxin
KFU87_RS05125 (1086836) 1086836..1087210 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KFU87_RS05130 (1087756) 1087756..1090905 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KFU87_RS05135 (1090928) 1090928..1092121 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T201108 WP_001291435.1 NZ_CP074019:c1086810-1086592 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T201108 NZ_CP098219:c1322523-1322305 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT201108 WP_000344800.1 NZ_CP074019:c1087210-1086836 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT201108 NZ_CP098219:c1322923-1322549 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References