Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 64880..65406 Replicon plasmid pYLPI24a
Accession NZ_CP074013
Organism Escherichia coli strain PI24

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag KFU81_RS23045 Protein ID WP_000323025.1
Coordinates 65119..65406 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q5J3S4
Locus tag KFU81_RS23040 Protein ID WP_000534857.1
Coordinates 64880..65119 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU81_RS23010 (KFU81_22870) 60705..61814 + 1110 WP_000842134.1 S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase -
KFU81_RS23015 (KFU81_22875) 61836..62258 + 423 Protein_60 alpha/beta hydrolase-fold protein -
KFU81_RS23020 (KFU81_22880) 62304..63008 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
KFU81_RS23025 (KFU81_22885) 63272..64345 - 1074 WP_000557466.1 hypothetical protein -
KFU81_RS23030 (KFU81_22890) 64389..64538 - 150 WP_000550473.1 hypothetical protein -
KFU81_RS23035 64745..64855 - 111 Protein_64 hypothetical protein -
KFU81_RS23040 (KFU81_22895) 64880..65119 + 240 WP_000534857.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
KFU81_RS23045 (KFU81_22900) 65119..65406 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
KFU81_RS23050 (KFU81_22905) 65710..66492 - 783 WP_001531258.1 IS21-like element ISSen3 family helper ATPase IstB -
KFU81_RS23055 (KFU81_22910) 66489..67511 - 1023 WP_000627495.1 IS21-like element ISSen3 family transposase -
KFU81_RS23060 (KFU81_22915) 67650..67874 + 225 Protein_69 hypothetical protein -
KFU81_RS23065 (KFU81_22920) 68480..69688 + 1209 WP_001339197.1 IS4-like element ISVsa5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(M) / dfrA16 / blaCARB-2 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / mef(B) / floR / tet(D) / aph(3')-IIa / aph(3'')-Ib / aph(6)-Id / tet(B) - 1..140432 140432
- inside IScluster/Tn tet(M) / dfrA16 / blaCARB-2 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / mef(B) / floR / tet(D) / aph(3')-IIa - 29748..84505 54757


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T201099 WP_000323025.1 NZ_CP074013:65119-65406 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T201099 NZ_CP098217:c4836142-4836035 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9085.55 Da        Isoelectric Point: 4.6820

>AT201099 WP_000534857.1 NZ_CP074013:64880-65119 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRKPKPVRVTLDEL

Download         Length: 240 bp

>AT201099 NZ_CP098217:4836190-4836256 [Escherichia coli]
GGTCTAGATGCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0NE60

References