Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 4162880..4163478 Replicon chromosome
Accession NZ_CP074012
Organism Escherichia coli strain PI24

Toxin (Protein)


Gene name doc Uniprot ID A0A8T6PRV3
Locus tag KFU81_RS20440 Protein ID WP_053291281.1
Coordinates 4162880..4163257 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID L4J1C0
Locus tag KFU81_RS20445 Protein ID WP_001603498.1
Coordinates 4163257..4163478 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU81_RS20410 (4158104) 4158104..4158442 + 339 WP_000883400.1 divalent cation tolerance protein CutA -
KFU81_RS20415 (4158418) 4158418..4160115 + 1698 WP_000068922.1 protein-disulfide reductase DsbD -
KFU81_RS20420 (4160152) 4160152..4160727 + 576 WP_001188520.1 transcriptional regulator -
KFU81_RS20430 (4161107) 4161107..4162369 + 1263 WP_096263470.1 integrase arm-type DNA-binding domain-containing protein -
KFU81_RS20435 (4162555) 4162555..4162770 + 216 Protein_3996 transposase -
KFU81_RS20440 (4162880) 4162880..4163257 - 378 WP_053291281.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KFU81_RS20445 (4163257) 4163257..4163478 - 222 WP_001603498.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KFU81_RS20460 (4166003) 4166003..4167871 + 1869 WP_223429133.1 DUF262 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4160152..4178372 18220
- inside IScluster/Tn - - 4162555..4178103 15548


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(4-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13487.28 Da        Isoelectric Point: 6.2257

>T201098 WP_053291281.1 NZ_CP074012:c4163257-4162880 [Escherichia coli]
MRHISPEELIAIHDANISRYGGLPGMPDSGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVYDSPELAELTVGAATGEVSVSTVVATLRRLYGTA

Download         Length: 378 bp

>T201098 NZ_CP098217:c4835659-4835552 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CATGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8190.24 Da        Isoelectric Point: 6.3159

>AT201098 WP_001603498.1 NZ_CP074012:c4163478-4163257 [Escherichia coli]
MQSINFRTARSNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDTTNKKLVNR

Download         Length: 222 bp

>AT201098 NZ_CP098217:4835707-4835773 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB L4J1C0

References