Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3947413..3947989 Replicon chromosome
Accession NZ_CP074012
Organism Escherichia coli strain PI24

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag KFU81_RS19310 Protein ID WP_001297643.1
Coordinates 3947702..3947989 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag KFU81_RS19305 Protein ID WP_000063156.1
Coordinates 3947413..3947715 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU81_RS19290 (3944009) 3944009..3946159 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
KFU81_RS19295 (3946209) 3946209..3946412 + 204 WP_000467859.1 YbdD/YjiX family protein -
KFU81_RS19300 (3946423) 3946423..3947379 + 957 WP_023277321.1 GTPase -
KFU81_RS19305 (3947413) 3947413..3947715 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
KFU81_RS19310 (3947702) 3947702..3947989 - 288 WP_001297643.1 BrnT family toxin Toxin
KFU81_RS19315 (3948188) 3948188..3948885 - 698 Protein_3777 IS1 family transposase -
KFU81_RS19320 (3948942) 3948942..3949037 + 96 Protein_3778 dienelactone hydrolase family protein -
KFU81_RS19325 (3949183) 3949183..3950415 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
KFU81_RS19330 (3950456) 3950456..3951736 + 1281 WP_130570461.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3948188..3948397 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T201096 WP_001297643.1 NZ_CP074012:c3947989-3947702 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T201096 NZ_CP098217:4216706-4217083 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGTACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT201096 WP_000063156.1 NZ_CP074012:c3947715-3947413 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT201096 NZ_CP098217:4216248-4216616 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTAACAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References