Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yafW-ykfI/CbtA-CbeA |
| Location | 3618676..3619355 | Replicon | chromosome |
| Accession | NZ_CP074012 | ||
| Organism | Escherichia coli strain PI24 | ||
Toxin (Protein)
| Gene name | ykfI | Uniprot ID | S1FHS4 |
| Locus tag | KFU81_RS17740 | Protein ID | WP_000854680.1 |
| Coordinates | 3619014..3619355 (+) | Length | 114 a.a. |
Antitoxin (Protein)
| Gene name | yafW | Uniprot ID | S1EWR7 |
| Locus tag | KFU81_RS17735 | Protein ID | WP_000070396.1 |
| Coordinates | 3618676..3618993 (+) | Length | 106 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KFU81_RS17690 (3614240) | 3614240..3614935 | + | 696 | Protein_3463 | DUF945 domain-containing protein | - |
| KFU81_RS17695 (3615152) | 3615152..3615853 | + | 702 | WP_021574425.1 | WYL domain-containing protein | - |
| KFU81_RS17700 (3615894) | 3615894..3616130 | + | 237 | WP_001144031.1 | protein YpjK | - |
| KFU81_RS17705 (3616130) | 3616130..3616567 | + | 438 | WP_016154551.1 | lipoprotein YafY | - |
| KFU81_RS17710 (3616596) | 3616596..3617063 | + | 468 | WP_001547765.1 | protein YkfB | - |
| KFU81_RS17715 (3617140) | 3617140..3617379 | + | 240 | WP_000194654.1 | DUF905 family protein | - |
| KFU81_RS17720 (3617477) | 3617477..3617935 | + | 459 | WP_000211838.1 | antirestriction protein | - |
| KFU81_RS17725 (3617951) | 3617951..3618427 | + | 477 | WP_000811693.1 | RadC family protein | - |
| KFU81_RS17730 (3618436) | 3618436..3618657 | + | 222 | WP_000691994.1 | DUF987 domain-containing protein | - |
| KFU81_RS17735 (3618676) | 3618676..3618993 | + | 318 | WP_000070396.1 | type IV toxin-antitoxin system antitoxin YafW | Antitoxin |
| KFU81_RS17740 (3619014) | 3619014..3619355 | + | 342 | WP_000854680.1 | type IV toxin-antitoxin system toxin YkfI | Toxin |
| KFU81_RS17750 (3619927) | 3619927..3621180 | - | 1254 | WP_000893266.1 | glutamate-5-semialdehyde dehydrogenase | - |
| KFU81_RS17755 (3621192) | 3621192..3622295 | - | 1104 | WP_001285288.1 | glutamate 5-kinase | - |
| KFU81_RS17760 (3622583) | 3622583..3623638 | + | 1056 | WP_219371408.1 | phosphoporin PhoE | - |
| KFU81_RS17765 (3623677) | 3623677..3624078 | - | 402 | WP_000174677.1 | sigma factor-binding protein Crl | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 12892.95 Da Isoelectric Point: 9.6543
>T201093 WP_000854680.1 NZ_CP074012:3619014-3619355 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
Download Length: 342 bp
>T201093 NZ_CP098217:3825411-3825722 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCTAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCTAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA
Antitoxin
Download Length: 106 a.a. Molecular weight: 11982.62 Da Isoelectric Point: 7.0270
>AT201093 WP_000070396.1 NZ_CP074012:3618676-3618993 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
Download Length: 318 bp
>AT201093 NZ_CP098217:3825719-3826138 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCCTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCATATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCCTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCATATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|