Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3417794..3418412 Replicon chromosome
Accession NZ_CP074012
Organism Escherichia coli strain PI24

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KFU81_RS16720 Protein ID WP_001291435.1
Coordinates 3418194..3418412 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KFU81_RS16715 Protein ID WP_000344800.1
Coordinates 3417794..3418168 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU81_RS16705 (3412883) 3412883..3414076 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KFU81_RS16710 (3414099) 3414099..3417248 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KFU81_RS16715 (3417794) 3417794..3418168 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KFU81_RS16720 (3418194) 3418194..3418412 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KFU81_RS16725 (3418584) 3418584..3419135 + 552 WP_000102564.1 maltose O-acetyltransferase -
KFU81_RS16730 (3419251) 3419251..3419721 + 471 WP_000136192.1 YlaC family protein -
KFU81_RS16735 (3419885) 3419885..3421435 + 1551 WP_021553404.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KFU81_RS16740 (3421477) 3421477..3421830 - 354 WP_000878140.1 DUF1428 domain-containing protein -
KFU81_RS16750 (3422209) 3422209..3422520 + 312 WP_000409911.1 MGMT family protein -
KFU81_RS16755 (3422551) 3422551..3423123 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T201092 WP_001291435.1 NZ_CP074012:3418194-3418412 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T201092 NZ_CP098217:c3100202-3099951 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT201092 WP_000344800.1 NZ_CP074012:3417794-3418168 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT201092 NZ_CP098217:c3099954-3099700 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References