Detailed information of TA system TA201091

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2470779..2471417
• Replicon: chromosome
• Accession: NZ_CP074012
• Organism: Escherichia coli strain PI24

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: KFU81_RS12160
• Protein ID: WP_000813794.1
• Coordinates: 2471241..2471417 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KFU81_RS12155
• Protein ID: WP_001270286.1
• Coordinates: 2470779..2471195 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU81_RS12135 (2465931)	2465931..2466872	-	942	WP_032177241.1	ABC transporter permease	-
KFU81_RS12140 (2466873)	2466873..2467886	-	1014	WP_032177240.1	ABC transporter ATP-binding protein	-
KFU81_RS12145 (2467904)	2467904..2469049	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
KFU81_RS12150 (2469294)	2469294..2470700	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
KFU81_RS12155 (2470779)	2470779..2471195	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KFU81_RS12160 (2471241)	2471241..2471417	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KFU81_RS12165 (2471639)	2471639..2471869	+	231	WP_000494244.1	YncJ family protein	-
KFU81_RS12170 (2471961)	2471961..2473922	-	1962	WP_001515270.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KFU81_RS12175 (2473995)	2473995..2474531	-	537	WP_000429150.1	DNA-binding transcriptional regulator SutR	-
KFU81_RS12180 (2474623)	2474623..2475798	+	1176	WP_053883748.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2475838..2476986	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T201091 WP_000813794.1 NZ_CP074012:c2471417-2471241 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T201091 NZ_CP098217:c3099954-3099700 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT201091 WP_001270286.1 NZ_CP074012:c2471195-2470779 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT201091 NZ_CP098217:c3100202-3099951 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• PDB: 6HPB