Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 749342..750177 Replicon chromosome
Accession NZ_CP074012
Organism Escherichia coli strain PI24

Toxin (Protein)


Gene name yeeV Uniprot ID A0A7W4PV54
Locus tag KFU81_RS03640 Protein ID WP_000854821.1
Coordinates 749800..750177 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A4Q1HU91
Locus tag KFU81_RS03635 Protein ID WP_053287563.1
Coordinates 749342..749710 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU81_RS03605 (745309) 745309..746181 + 873 WP_053292456.1 GTPase family protein -
KFU81_RS03610 (746350) 746350..746760 + 411 Protein_709 autotransporter adhesin family protein -
KFU81_RS03615 (747087) 747087..747905 + 819 WP_053292454.1 DUF932 domain-containing protein -
KFU81_RS03620 (747996) 747996..748481 + 486 WP_000214317.1 antirestriction protein -
KFU81_RS03625 (748496) 748496..748972 + 477 WP_001186747.1 RadC family protein -
KFU81_RS03630 (749041) 749041..749262 + 222 WP_000692309.1 DUF987 domain-containing protein -
KFU81_RS03635 (749342) 749342..749710 + 369 WP_053287563.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
KFU81_RS03640 (749800) 749800..750177 + 378 WP_000854821.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
KFU81_RS03645 (750174) 750174..750371 + 198 Protein_716 DUF5983 family protein -
KFU81_RS03650 (750399) 750399..750596 + 198 WP_088209831.1 DUF957 domain-containing protein -
KFU81_RS03655 (750681) 750681..751526 + 846 WP_063101552.1 DUF4942 domain-containing protein -
KFU81_RS03665 (751825) 751825..752331 + 507 WP_000228937.1 G/U mismatch-specific DNA glycosylase -
KFU81_RS03670 (752410) 752410..754251 - 1842 WP_000437375.1 RNA polymerase sigma factor RpoD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13930.95 Da        Isoelectric Point: 8.5163

>T201078 WP_000854821.1 NZ_CP074012:749800-750177 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Download         Length: 378 bp

>T201078 NZ_CP098217:639636-639926 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13663.36 Da        Isoelectric Point: 6.4768

>AT201078 WP_053287563.1 NZ_CP074012:749342-749710 [Escherichia coli]
VSDTFSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNSRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Download         Length: 369 bp

>AT201078 NZ_CP098217:639404-639646 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W4PV54

Antitoxin

Source ID Structure
AlphaFold DB A0A4Q1HU91

References