Detailed information of TA system TA201075

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 71298..71833
• Replicon: plasmid pYLPD4b
• Accession: NZ_CP074009
• Organism: Escherichia coli strain PD4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KFU74_RS24140
• Protein ID: WP_032206513.1
• Coordinates: 71298..71585 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: T1PXV2
• Locus tag: KFU74_RS24145
• Protein ID: WP_001139206.1
• Coordinates: 71582..71833 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU74_RS24125 (KFU74_23965)	66923..70438	+	3516	WP_072752622.1	autotransporter outer membrane beta-barrel domain-containing protein	-
KFU74_RS24130 (KFU74_23970)	70613..70818	-	206	Protein_90	hypothetical protein	-
KFU74_RS24135	70817..70930	+	114	Protein_91	alkaline phosphatase	-
KFU74_RS24140 (KFU74_23980)	71298..71585	-	288	WP_032206513.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KFU74_RS24145 (KFU74_23985)	71582..71833	-	252	WP_001139206.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KFU74_RS24150 (KFU74_23990)	72415..72807	+	393	Protein_94	hypothetical protein	-
KFU74_RS24155 (KFU74_23995)	72838..75804	-	2967	WP_001138014.1	Tn3-like element TnAs1 family transposase	-
KFU74_RS24160 (KFU74_24000)	75808..76368	-	561	WP_001161490.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aadA5 / sul2	-	1..84156	84156

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11130.20 Da
• Isoelectric Point: 10.5833

>T201075 WP_032206513.1 NZ_CP074009:c71585-71298 [Escherichia coli]
MTYTVKFRDDALKEWLRLDKTIQQQFAKKLKKCSENPHIASAKLRGLKDCYKIKLRASGFRLVYQIIDDMLIIAVVAVGK
RERSNVYHLASERMK

Nucleotide

• Length: 288 bp

>T201075 NZ_CP098217:222931-223242 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9171.39 Da
• Isoelectric Point: 4.0927

>AT201075 WP_001139206.1 NZ_CP074009:c71833-71582 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSSQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT201075 NZ_CP098217:223243-223533 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Antitoxin

• AlphaFold DB: T1PXV2