Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 16892..17493 Replicon plasmid pYLPD4b
Accession NZ_CP074009
Organism Escherichia coli strain PD4

Toxin (Protein)


Gene name doc Uniprot ID F0JYA5
Locus tag KFU74_RS23790 Protein ID WP_001216044.1
Coordinates 16892..17272 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag KFU74_RS23795 Protein ID WP_001190712.1
Coordinates 17272..17493 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU74_RS23755 (KFU74_23605) 12870..13091 - 222 WP_000542336.1 hypothetical protein -
KFU74_RS23765 (KFU74_23615) 14465..14674 + 210 WP_000874154.1 hypothetical protein -
KFU74_RS23770 (KFU74_23620) 14785..15636 + 852 WP_000611653.1 phage repressor protein C1 -
KFU74_RS23775 15669..15794 - 126 WP_257233066.1 hypothetical protein -
KFU74_RS23780 (KFU74_23625) 15811..16680 - 870 Protein_20 DUF968 domain-containing protein -
KFU74_RS23785 (KFU74_23630) 16708..16887 - 180 WP_039022005.1 hypothetical protein -
KFU74_RS23790 (KFU74_23635) 16892..17272 - 381 WP_001216044.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KFU74_RS23795 (KFU74_23640) 17272..17493 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KFU74_RS23800 (KFU74_23645) 17566..17955 - 390 WP_000506730.1 S24 family peptidase -
KFU74_RS23805 (KFU74_23650) 18130..18705 + 576 WP_039022006.1 hypothetical protein -
KFU74_RS23810 (KFU74_23655) 18712..18963 - 252 WP_039022007.1 DNA polymerase III subunit theta -
KFU74_RS23815 (KFU74_23660) 19753..20019 - 267 WP_223671585.1 hypothetical protein -
KFU74_RS23820 (KFU74_23665) 20089..20838 - 750 WP_155057219.1 hypothetical protein -
KFU74_RS23825 (KFU74_23670) 20820..21194 - 375 WP_039022008.1 hypothetical protein -
KFU74_RS23830 (KFU74_23675) 21261..21425 - 165 WP_152950163.1 hypothetical protein -
KFU74_RS23835 (KFU74_23680) 21425..21718 - 294 WP_039022009.1 hypothetical protein -
KFU74_RS23840 22068..22247 - 180 Protein_32 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aadA5 / sul2 - 1..84156 84156
- flank IS/Tn - - 13999..14274 275


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13544.28 Da        Isoelectric Point: 5.6343

>T201074 WP_001216044.1 NZ_CP074009:c17272-16892 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVAATLRRLYGSAE

Download         Length: 381 bp

>T201074 NZ_CP098215:107669-107818 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT201074 WP_001190712.1 NZ_CP074009:c17493-17272 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT201074 NZ_CP098215:c107625-107564 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6Y6HMN5

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References