Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4364144..4364720 Replicon chromosome
Accession NZ_CP074007
Organism Escherichia coli strain PD4

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag KFU74_RS20920 Protein ID WP_001297643.1
Coordinates 4364433..4364720 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag KFU74_RS20915 Protein ID WP_000063156.1
Coordinates 4364144..4364446 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU74_RS20900 (4360740) 4360740..4362890 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
KFU74_RS20905 (4362940) 4362940..4363143 + 204 WP_000467859.1 YbdD/YjiX family protein -
KFU74_RS20910 (4363154) 4363154..4364110 + 957 WP_001297640.1 GTPase -
KFU74_RS20915 (4364144) 4364144..4364446 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
KFU74_RS20920 (4364433) 4364433..4364720 - 288 WP_001297643.1 BrnT family toxin Toxin
KFU74_RS20930 (4365673) 4365673..4365768 + 96 Protein_4081 dienelactone hydrolase family protein -
KFU74_RS20935 (4365914) 4365914..4367146 + 1233 WP_024186283.1 multidrug efflux MFS transporter MdtM -
KFU74_RS20940 (4367187) 4367187..4368467 + 1281 WP_001037400.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T201071 WP_001297643.1 NZ_CP074007:c4364720-4364433 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T201071 NZ_CP098214:c4588286-4588113 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT201071 WP_000063156.1 NZ_CP074007:c4364446-4364144 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT201071 NZ_CP098214:c4588610-4588314 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References