Detailed information of TA system TA201068

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3979837..3980635
• Replicon: chromosome
• Accession: NZ_CP074007
• Organism: Escherichia coli strain PD4

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A166U9J9
• Locus tag: KFU74_RS19115
• Protein ID: WP_000854695.1
• Coordinates: 3979837..3980214 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: KFU74_RS19120
• Protein ID: WP_191007491.1
• Coordinates: 3980261..3980635 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU74_RS19085 (3975115)	3975115..3976071	+	957	WP_000121346.1	molybdenum cofactor insertion chaperone PaoD	-
KFU74_RS19090 (3976122)	3976122..3976460	+	339	Protein_3724	LysR substrate-binding domain-containing protein	-
KFU74_RS19095 (3976798)	3976798..3978117	+	1320	WP_191007492.1	site-specific integrase	-
KFU74_RS19100 (3978210)	3978210..3979058	-	849	WP_001280536.1	DUF4942 domain-containing protein	-
KFU74_RS19105 (3979143)	3979143..3979340	-	198	WP_000839228.1	DUF957 domain-containing protein	-
KFU74_RS19110 (3979352)	3979352..3979840	-	489	WP_000761710.1	DUF5983 family protein	-
KFU74_RS19115 (3979837)	3979837..3980214	-	378	WP_000854695.1	TA system toxin CbtA family protein	Toxin
KFU74_RS19120 (3980261)	3980261..3980635	-	375	WP_191007491.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KFU74_RS19125 (3980798)	3980798..3981019	-	222	WP_000692307.1	DUF987 domain-containing protein	-
KFU74_RS19130 (3981088)	3981088..3981564	-	477	WP_001186773.1	RadC family protein	-
KFU74_RS19135 (3981580)	3981580..3982059	-	480	WP_000860084.1	antirestriction protein	-
KFU74_RS19140 (3982141)	3982141..3982959	-	819	WP_001241366.1	DUF932 domain-containing protein	-
KFU74_RS19145 (3983298)	3983298..3985070	-	1773	Protein_3735	autotransporter adhesin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3948956..3982059	33103

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14062.03 Da
• Isoelectric Point: 7.7531

>T201068 WP_000854695.1 NZ_CP074007:c3980214-3979837 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPGAKQ

Nucleotide

• Length: 378 bp

>T201068 NZ_CP098214:4211670-4212011 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACTGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13840.69 Da
• Isoelectric Point: 7.0266

>AT201068 WP_191007491.1 NZ_CP074007:c3980635-3980261 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCETDTLGSCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT201068 NZ_CP098214:c4211675-4211599 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A166U9J9