Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1317355..1317938 Replicon chromosome
Accession NZ_CP074007
Organism Escherichia coli strain PD4

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag KFU74_RS06385 Protein ID WP_000254745.1
Coordinates 1317603..1317938 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag KFU74_RS06380 Protein ID WP_000581937.1
Coordinates 1317355..1317603 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU74_RS06370 (1313694) 1313694..1314995 + 1302 WP_000046785.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
KFU74_RS06375 (1315043) 1315043..1317277 + 2235 WP_000226815.1 GTP pyrophosphokinase -
KFU74_RS06380 (1317355) 1317355..1317603 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KFU74_RS06385 (1317603) 1317603..1317938 + 336 WP_000254745.1 endoribonuclease MazF Toxin
KFU74_RS06390 (1318009) 1318009..1318800 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
KFU74_RS06395 (1319028) 1319028..1320665 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
KFU74_RS06400 (1320753) 1320753..1322051 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T201053 WP_000254745.1 NZ_CP074007:1317603-1317938 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T201053 NZ_CP098214:338321-338428 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT201053 WP_000581937.1 NZ_CP074007:1317355-1317603 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT201053 NZ_CP098214:c338273-338207 [Escherichia coli]
GGTCTAGGTTCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References