Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1168668..1169322 Replicon chromosome
Accession NZ_CP074007
Organism Escherichia coli strain PD4

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag KFU74_RS05700 Protein ID WP_000244772.1
Coordinates 1168915..1169322 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KFU74_RS05695 Protein ID WP_000354046.1
Coordinates 1168668..1168934 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU74_RS05670 (1163956) 1163956..1164699 + 744 WP_000951964.1 SDR family oxidoreductase -
KFU74_RS05675 (1164756) 1164756..1166189 - 1434 WP_024198262.1 6-phospho-beta-glucosidase BglA -
KFU74_RS05680 (1166234) 1166234..1166545 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
KFU74_RS05685 (1166709) 1166709..1167368 + 660 WP_000250274.1 hemolysin III family protein -
KFU74_RS05690 (1167445) 1167445..1168425 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KFU74_RS05695 (1168668) 1168668..1168934 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KFU74_RS05700 (1168915) 1168915..1169322 + 408 WP_000244772.1 protein YgfX Toxin
KFU74_RS05705 (1169362) 1169362..1169883 - 522 WP_001055874.1 flavodoxin FldB -
KFU74_RS05710 (1169995) 1169995..1170891 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KFU74_RS05715 (1170916) 1170916..1171626 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KFU74_RS05720 (1171632) 1171632..1173365 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T201052 WP_000244772.1 NZ_CP074007:1168915-1169322 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T201052 NZ_CP098214:c87906-87529 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCAGGGTAAGTATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT201052 WP_000354046.1 NZ_CP074007:1168668-1168934 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT201052 NZ_CP098214:c88327-87953 [Escherichia coli]
GTGTCAGACACACTCCCTGGGACAACGCTTCCCGACGACAATAACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAAGGGAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGGCCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References