Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3969252..3969828 Replicon chromosome
Accession NZ_CP074005
Organism Escherichia coli strain PI12

Toxin (Protein)


Gene name shpA Uniprot ID A0A0E2LC92
Locus tag KFU69_RS19270 Protein ID WP_001299718.1
Coordinates 3969541..3969828 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A7U9DAW4
Locus tag KFU69_RS19265 Protein ID WP_000063150.1
Coordinates 3969252..3969554 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU69_RS19250 (3965848) 3965848..3967998 + 2151 WP_134264336.1 pyruvate/proton symporter BtsT -
KFU69_RS19255 (3968048) 3968048..3968251 + 204 WP_000467859.1 YbdD/YjiX family protein -
KFU69_RS19260 (3968262) 3968262..3969218 + 957 WP_098704077.1 GTPase -
KFU69_RS19265 (3969252) 3969252..3969554 - 303 WP_000063150.1 BrnA antitoxin family protein Antitoxin
KFU69_RS19270 (3969541) 3969541..3969828 - 288 WP_001299718.1 BrnT family toxin Toxin
KFU69_RS19275 (3970025) 3970025..3971191 + 1167 WP_098704076.1 restriction endonuclease -
KFU69_RS19280 (3971255) 3971255..3972874 + 1620 WP_098704075.1 class I SAM-dependent DNA methyltransferase -
KFU69_RS19285 (3972864) 3972864..3973433 + 570 WP_152933965.1 restriction endonuclease subunit S -
KFU69_RS19295 (3974658) 3974658..3974756 + 99 Protein_3774 type I restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11142.60 Da        Isoelectric Point: 7.4697

>T201049 WP_001299718.1 NZ_CP074005:c3969828-3969541 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGVVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T201049 NZ_CP098211:c4405691-4405518 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11532.03 Da        Isoelectric Point: 9.4646

>AT201049 WP_000063150.1 NZ_CP074005:c3969554-3969252 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT201049 NZ_CP098211:c4406015-4405719 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E2LC92

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DAW4

References