Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3492113..3492731 Replicon chromosome
Accession NZ_CP074005
Organism Escherichia coli strain PI12

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KFU69_RS16975 Protein ID WP_001291435.1
Coordinates 3492513..3492731 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KFU69_RS16970 Protein ID WP_000344800.1
Coordinates 3492113..3492487 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU69_RS16960 (3487202) 3487202..3488395 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KFU69_RS16965 (3488418) 3488418..3491567 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KFU69_RS16970 (3492113) 3492113..3492487 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KFU69_RS16975 (3492513) 3492513..3492731 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KFU69_RS16980 (3492903) 3492903..3493454 + 552 WP_000102564.1 maltose O-acetyltransferase -
KFU69_RS16985 (3493570) 3493570..3494040 + 471 WP_000136192.1 YlaC family protein -
KFU69_RS16990 (3494204) 3494204..3495754 + 1551 WP_161992032.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KFU69_RS16995 (3495796) 3495796..3496149 - 354 WP_000878140.1 DUF1428 domain-containing protein -
KFU69_RS17005 (3496528) 3496528..3496839 + 312 WP_000409911.1 MGMT family protein -
KFU69_RS17010 (3496870) 3496870..3497442 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T201047 WP_001291435.1 NZ_CP074005:3492513-3492731 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T201047 NZ_CP098211:c4233914-4233639 [Escherichia coli]
ATGGAAATTTACTGGACGTTTAAAGCTCAGGATGACCTAGAACGTATCTATCGTTTCGCCCTTCAGTATAGCCGCAAGCA
CGCCGATGACATTTTGGATCGCCTAATTACTGGCTGCGCTGGCCTTACAGCGCATCCGGCAATTGGTGTACATCAGACTC
GTTATGAACCCCGAGAAGTCCGAAAAGTGTTATTTGATGACTATGAGGTTCACTACGAAATCCGTGACAATGATATCCAT
ATCGTGGATCTCTGGCATACAAAAGAAGAACGATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT201047 WP_000344800.1 NZ_CP074005:3492113-3492487 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT201047 NZ_CP098211:c4234166-4233918 [Escherichia coli]
ATGGGCTCACTGACTAAACAAACCATCAGCGCACAGATACCCGTTGAGTTGGCTGCTGCTGTTGAGAATCTGGCAATTGA
GTTAGATCGCTCTAAAAGCTGGATTATTAAGGAAGCGTTGACCTGTATGCTGGCCGAAAGAGAGCGGCGTCACCAGAGCA
TCCAGGCCGGTCTTGCCGATGTGGATGCGGGTCGCGTGGTGAGCCACTCAGACATGGTGGATTTTGCTAATCGCTTAAAG
AAAGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References