Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/ParE-RelB |
| Location | 103765..104291 | Replicon | plasmid pYLPM4a |
| Accession | NZ_CP074002 | ||
| Organism | Escherichia coli strain PM4 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | S1EXL1 |
| Locus tag | KFU80_RS23155 | Protein ID | WP_000323025.1 |
| Coordinates | 103765..104052 (-) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | Q5J3S4 |
| Locus tag | KFU80_RS23160 | Protein ID | WP_000534857.1 |
| Coordinates | 104052..104291 (-) | Length | 80 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KFU80_RS23135 (KFU80_22930) | 99483..100691 | - | 1209 | WP_001339197.1 | IS4-like element ISVsa5 family transposase | - |
| KFU80_RS23140 (KFU80_22935) | 101297..101521 | - | 225 | Protein_103 | hypothetical protein | - |
| KFU80_RS23145 (KFU80_22940) | 101660..102682 | + | 1023 | WP_000627495.1 | IS21-like element ISSen3 family transposase | - |
| KFU80_RS23150 (KFU80_22945) | 102679..103461 | + | 783 | WP_001531258.1 | IS21-like element ISSen3 family helper ATPase IstB | - |
| KFU80_RS23155 (KFU80_22950) | 103765..104052 | - | 288 | WP_000323025.1 | type II toxin-antitoxin system mRNA interferase RelE | Toxin |
| KFU80_RS23160 (KFU80_22955) | 104052..104291 | - | 240 | WP_000534857.1 | type II toxin-antitoxin system antitoxin RelB | Antitoxin |
| KFU80_RS23165 | 104316..104426 | + | 111 | Protein_108 | hypothetical protein | - |
| KFU80_RS23170 (KFU80_22960) | 104633..104782 | + | 150 | WP_000550473.1 | hypothetical protein | - |
| KFU80_RS23175 (KFU80_22965) | 104826..105899 | + | 1074 | WP_000557466.1 | hypothetical protein | - |
| KFU80_RS23180 (KFU80_22970) | 106163..106867 | + | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| KFU80_RS23185 (KFU80_22975) | 106919..107191 | - | 273 | Protein_112 | copper resistance inner membrane protein PcoD | - |
| KFU80_RS23190 (KFU80_22980) | 107196..107576 | - | 381 | WP_000025662.1 | copper resistance system metallochaperone PcoC | - |
| KFU80_RS23195 (KFU80_22985) | 107616..108506 | - | 891 | WP_001378118.1 | copper resistance outer membrane transporter PcoB | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | tet(M) / dfrA16 / blaCARB-2 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / mef(B) / tet(B) / aph(6)-Id / aph(3'')-Ib / aph(3')-IIa | - | 1..136494 | 136494 | |
| - | inside | IScluster/Tn | aph(3')-IIa | - | 84666..103461 | 18795 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11225.22 Da Isoelectric Point: 10.1967
>T201029 WP_000323025.1 NZ_CP074002:c104052-103765 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL
Download Length: 288 bp
>T201029 NZ_CP098210:c4846869-4846684 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA
Antitoxin
Download Length: 80 a.a. Molecular weight: 9085.55 Da Isoelectric Point: 4.6820
>AT201029 WP_000534857.1 NZ_CP074002:c104291-104052 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRKPKPVRVTLDEL
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRKPKPVRVTLDEL
Download Length: 240 bp
>AT201029 NZ_CP098210:c4846667-4846257 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGTGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGGTTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCAGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGTGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGGTTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCAGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|