Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4193920..4194417 Replicon chromosome
Accession NZ_CP074001
Organism Escherichia coli strain PM4

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KFU80_RS20495 Protein ID WP_001173343.1
Coordinates 4193920..4194093 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KFU80_RS20500 Protein ID WP_000398619.1
Coordinates 4194121..4194417 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU80_RS20485 (4190590) 4190590..4192047 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
KFU80_RS20490 (4192284) 4192284..4193801 + 1518 WP_001295074.1 lysine--tRNA ligase -
KFU80_RS20495 (4193920) 4193920..4194093 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KFU80_RS20500 (4194121) 4194121..4194417 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KFU80_RS20505 (4194644) 4194644..4194916 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
KFU80_RS20510 (4194928) 4194928..4195158 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
KFU80_RS20515 (4195339) 4195339..4196970 + 1632 WP_001216477.1 sensor histidine kinase -
KFU80_RS20520 (4196967) 4196967..4197686 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T201027 WP_001173343.1 NZ_CP074001:c4194093-4193920 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T201027 NZ_CP098210:c4790700-4790593 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT201027 WP_000398619.1 NZ_CP074001:c4194417-4194121 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT201027 NZ_CP098210:4790748-4790814 [Escherichia coli]
GGTCTAGATGCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References