Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3410444..3411062 Replicon chromosome
Accession NZ_CP074001
Organism Escherichia coli strain PM4

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KFU80_RS16555 Protein ID WP_001291435.1
Coordinates 3410844..3411062 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KFU80_RS16550 Protein ID WP_000344800.1
Coordinates 3410444..3410818 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU80_RS16540 (3405533) 3405533..3406726 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KFU80_RS16545 (3406749) 3406749..3409898 + 3150 WP_016241120.1 efflux RND transporter permease AcrB -
KFU80_RS16550 (3410444) 3410444..3410818 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KFU80_RS16555 (3410844) 3410844..3411062 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KFU80_RS16560 (3411234) 3411234..3411785 + 552 WP_000102564.1 maltose O-acetyltransferase -
KFU80_RS16565 (3411901) 3411901..3412371 + 471 WP_000136192.1 YlaC family protein -
KFU80_RS16570 (3412535) 3412535..3414085 + 1551 WP_130562191.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KFU80_RS16575 (3414127) 3414127..3414480 - 354 WP_000878140.1 DUF1428 domain-containing protein -
KFU80_RS16585 (3414859) 3414859..3415170 + 312 WP_000409911.1 MGMT family protein -
KFU80_RS16590 (3415201) 3415201..3415773 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T201023 WP_001291435.1 NZ_CP074001:3410844-3411062 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T201023 NZ_CP098210:c3929945-3929025 [Escherichia coli]
ATGGATCTGCGTGATAGCTATGCCCGGCAGGTCAAACTGTTGATGGCCGCTCTGCCGTATGTGGCGAAGGAGTCCTGTTT
CGCGTTGAAAGGCGGCACCGCAATCAATTTGTTTGTGCAGGATTTTCCCCGTCTGTCGGTGGATATTGATCTGGCGTACA
AGAGCTTTGCGGATCGCGATACTGATTTGGCGGCTATCAACGACGCTTTGAAGAGGATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATCTGCGCTGCGCTTGATCGTCAGCAC
CCGCGCGATCTGTTTGATGTGCTGAATATGCTGGAGAAACCGGGACTGATGCGGGAGATCTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGCCCCATCGCCGAGTTGCTGGCTCCAAACTGGGATACAGAGCGGATCGCCACGTTGTACGCGC
AGGAATTTTCAGGGATGACGCAACAGGAAACGTCGCTGGAATCGCTTCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATCTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGCAATCTGGCGGTGCTTAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTTCTGGCGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT201023 WP_000344800.1 NZ_CP074001:3410444-3410818 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT201023 NZ_CP098210:c3930714-3929932 [Escherichia coli]
TTGCCAAACTATCTAAACTGGCTGATGCAGCACACGCTACCCGGCCAGGTGCTGCTCCAGTCATGGCTTACCCGCAACGG
CATAGATCGTAAGCTCTCTTATCTCTATGTGAAGAATGGCTGGCTCAGGCGGATCGCTCACGGCGTATACTGCCGGACGG
GTCGTGAACCGGATTGGGTGGATGCGGTTTACTGCCTGCAATCGCAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGTCATGCGCACTATACCGAACAGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTTTCTGCCTTTGAACAATCCGCAACGTTTGTCACGCTGTATACGTCAGGCCTTACCGCTGATGTAA
CGCCATTTACTACGGAACTGAAAATTGGCGACCGCCAGTTAGCAGGCAGTGTTCCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTAATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGTTGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGCCATTCGGTTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATATTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCCGGCGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCACGCGCATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References