Detailed information of TA system TA201022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2409775..2410413
• Replicon: chromosome
• Accession: NZ_CP074001
• Organism: Escherichia coli strain PM4

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: KFU80_RS11735
• Protein ID: WP_000813794.1
• Coordinates: 2410237..2410413 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KFU80_RS11730
• Protein ID: WP_001270286.1
• Coordinates: 2409775..2410191 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU80_RS11710 (2404927)	2404927..2405868	-	942	WP_001251313.1	ABC transporter permease	-
KFU80_RS11715 (2405869)	2405869..2406882	-	1014	WP_097740840.1	ABC transporter ATP-binding protein	-
KFU80_RS11720 (2406900)	2406900..2408045	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
KFU80_RS11725 (2408290)	2408290..2409696	-	1407	WP_000760585.1	PLP-dependent aminotransferase family protein	-
KFU80_RS11730 (2409775)	2409775..2410191	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KFU80_RS11735 (2410237)	2410237..2410413	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KFU80_RS11740 (2410635)	2410635..2410865	+	231	WP_000494244.1	YncJ family protein	-
KFU80_RS11745 (2410957)	2410957..2412918	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KFU80_RS11750 (2412991)	2412991..2413527	-	537	WP_000429133.1	DNA-binding transcriptional regulator SutR	-
KFU80_RS11755 (2413619)	2413619..2414794	+	1176	WP_001236251.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2414834..2416099	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T201022 WP_000813794.1 NZ_CP074001:c2410413-2410237 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T201022 NZ_CP098210:3724197-3724415 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT201022 WP_001270286.1 NZ_CP074001:c2410191-2409775 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT201022 NZ_CP098210:3723797-3724171 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• PDB: 6HPB