Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 814046..814700 Replicon chromosome
Accession NZ_CP074001
Organism Escherichia coli strain PM4

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KFU80_RS04035 Protein ID WP_000244777.1
Coordinates 814293..814700 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KFU80_RS04030 Protein ID WP_000354046.1
Coordinates 814046..814312 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU80_RS04005 (809215) 809215..809958 + 744 WP_094313880.1 SDR family oxidoreductase -
KFU80_RS04010 (810015) 810015..811448 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
KFU80_RS04015 (811493) 811493..811804 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
KFU80_RS04020 (811968) 811968..812627 + 660 WP_000250274.1 hemolysin III family protein -
KFU80_RS04025 (812823) 812823..813803 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KFU80_RS04030 (814046) 814046..814312 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KFU80_RS04035 (814293) 814293..814700 + 408 WP_000244777.1 protein YgfX Toxin
KFU80_RS04040 (814740) 814740..815261 - 522 WP_001055874.1 flavodoxin FldB -
KFU80_RS04045 (815373) 815373..816269 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KFU80_RS04050 (816294) 816294..817004 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KFU80_RS04055 (817010) 817010..818743 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T201010 WP_000244777.1 NZ_CP074001:814293-814700 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T201010 NZ_CP098210:566912-567262 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGTGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT201010 WP_000354046.1 NZ_CP074001:814046-814312 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT201010 NZ_CP098210:566667-566918 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References