Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4154468..4155086 Replicon chromosome
Accession NZ_CP073996
Organism Escherichia coli strain PM7

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KFU86_RS20165 Protein ID WP_001291435.1
Coordinates 4154468..4154686 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KFU86_RS20170 Protein ID WP_000344800.1
Coordinates 4154712..4155086 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU86_RS20130 (4149757) 4149757..4150329 + 573 WP_000779831.1 YbaY family lipoprotein -
KFU86_RS20135 (4150360) 4150360..4150671 - 312 WP_000409911.1 MGMT family protein -
KFU86_RS20145 (4151050) 4151050..4151403 + 354 WP_094337314.1 DUF1428 family protein -
KFU86_RS20150 (4151445) 4151445..4152995 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KFU86_RS20155 (4153159) 4153159..4153629 - 471 WP_000136192.1 YlaC family protein -
KFU86_RS20160 (4153745) 4153745..4154296 - 552 WP_000102564.1 maltose O-acetyltransferase -
KFU86_RS20165 (4154468) 4154468..4154686 - 219 WP_001291435.1 HHA domain-containing protein Toxin
KFU86_RS20170 (4154712) 4154712..4155086 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KFU86_RS20175 (4155632) 4155632..4158781 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KFU86_RS20180 (4158804) 4158804..4159997 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T201005 WP_001291435.1 NZ_CP073996:c4154686-4154468 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T201005 NZ_CP098206:c4860763-4860452 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT201005 WP_000344800.1 NZ_CP073996:c4155086-4154712 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT201005 NZ_CP098206:c4860451-4860161 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References