Detailed information of TA system TA200998

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 2087519..2088173
• Replicon: chromosome
• Accession: NZ_CP073996
• Organism: Escherichia coli strain PM7

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: S1EEB2
• Locus tag: KFU86_RS10145
• Protein ID: WP_000244777.1
• Coordinates: 2087519..2087926 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: KFU86_RS10150
• Protein ID: WP_257181194.1
• Coordinates: 2087907..2088173 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU86_RS10125 (2083476)	2083476..2085209	-	1734	WP_000813212.1	single-stranded-DNA-specific exonuclease RecJ	-
KFU86_RS10130 (2085215)	2085215..2085925	-	711	WP_000715214.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
KFU86_RS10135 (2085950)	2085950..2086846	-	897	WP_000806638.1	site-specific tyrosine recombinase XerD	-
KFU86_RS10140 (2086958)	2086958..2087479	+	522	WP_001055874.1	flavodoxin FldB	-
KFU86_RS10145 (2087519)	2087519..2087926	-	408	WP_000244777.1	protein YgfX	Toxin
KFU86_RS10150 (2087907)	2087907..2088173	-	267	WP_257181194.1	FAD assembly factor SdhE	Antitoxin
KFU86_RS10155 (2088416)	2088416..2089396	+	981	WP_000886062.1	tRNA-modifying protein YgfZ	-
KFU86_RS10160 (2089768)	2089768..2090748	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
KFU86_RS10165 (2090871)	2090871..2091530	-	660	WP_000250274.1	hemolysin III family protein	-
KFU86_RS10170 (2091694)	2091694..2092005	-	312	WP_001182947.1	N(4)-acetylcytidine aminohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2089768..2090748	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16031.96 Da
• Isoelectric Point: 11.5202

>T200998 WP_000244777.1 NZ_CP073996:c2087926-2087519 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T200998 NZ_CP098206:c3656604-3656302 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATTGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10575.17 Da
• Isoelectric Point: 5.1599

>AT200998 WP_257181194.1 NZ_CP073996:c2088173-2087907 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADVELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT200998 NZ_CP098206:c3656266-3655925 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• AlphaFold DB: A0A7U9LFV7