Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1961091..1961674 Replicon chromosome
Accession NZ_CP073996
Organism Escherichia coli strain PM7

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag KFU86_RS09615 Protein ID WP_000254745.1
Coordinates 1961091..1961426 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag KFU86_RS09620 Protein ID WP_000581937.1
Coordinates 1961426..1961674 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU86_RS09600 (1956978) 1956978..1958276 - 1299 WP_000036723.1 phosphopyruvate hydratase -
KFU86_RS09605 (1958364) 1958364..1960001 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
KFU86_RS09610 (1960229) 1960229..1961020 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
KFU86_RS09615 (1961091) 1961091..1961426 - 336 WP_000254745.1 endoribonuclease MazF Toxin
KFU86_RS09620 (1961426) 1961426..1961674 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KFU86_RS09625 (1961752) 1961752..1963986 - 2235 WP_000226815.1 GTP pyrophosphokinase -
KFU86_RS09630 (1964034) 1964034..1965335 - 1302 WP_000046800.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T200997 WP_000254745.1 NZ_CP073996:c1961426-1961091 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T200997 NZ_CP098206:2887397-2887504 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT200997 WP_000581937.1 NZ_CP073996:c1961674-1961426 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT200997 NZ_CP098206:c2887344-2887283 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References