Detailed information of TA system TA200996

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1672265..1672890
• Replicon: chromosome
• Accession: NZ_CP073996
• Organism: Escherichia coli strain PM7

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KFU86_RS08200
• Protein ID: WP_000911330.1
• Coordinates: 1672265..1672663 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: KFU86_RS08205
• Protein ID: WP_000450524.1
• Coordinates: 1672663..1672890 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU86_RS08180 (1668172)	1668172..1668372	+	201	WP_000383836.1	YpfN family protein	-
KFU86_RS08185 (1668453)	1668453..1669151	-	699	WP_000679812.1	esterase	-
KFU86_RS08190 (1669225)	1669225..1671240	-	2016	WP_033885019.1	tRNA cytosine(34) acetyltransferase TmcA	-
KFU86_RS08195 (1671255)	1671255..1672118	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
KFU86_RS08200 (1672265)	1672265..1672663	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
KFU86_RS08205 (1672663)	1672663..1672890	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
KFU86_RS08210 (1673044)	1673044..1673757	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
KFU86_RS08215 (1673970)	1673970..1675004	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
KFU86_RS08220 (1675021)	1675021..1675899	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
KFU86_RS08225 (1676045)	1676045..1676617	+	573	WP_000176192.1	glycine cleavage system transcriptional repressor	-
KFU86_RS08230 (1676617)	1676617..1677087	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T200996 WP_000911330.1 NZ_CP073996:c1672663-1672265 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T200996 NZ_CP098206:2611397-2611675 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT200996 WP_000450524.1 NZ_CP073996:c1672890-1672663 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT200996 NZ_CP098206:2611675-2611959 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33