Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 542007..542645 Replicon chromosome
Accession NZ_CP073996
Organism Escherichia coli strain PM7

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag KFU86_RS02665 Protein ID WP_000813794.1
Coordinates 542007..542183 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag KFU86_RS02670 Protein ID WP_001270286.1
Coordinates 542229..542645 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU86_RS02645 (537626) 537626..538801 - 1176 WP_001236265.1 BenE family transporter YdcO -
KFU86_RS02650 (538893) 538893..539429 + 537 WP_257180331.1 DNA-binding transcriptional regulator SutR -
KFU86_RS02655 (539502) 539502..541463 + 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
KFU86_RS02660 (541555) 541555..541785 - 231 WP_000494244.1 YncJ family protein -
KFU86_RS02665 (542007) 542007..542183 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
KFU86_RS02670 (542229) 542229..542645 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
KFU86_RS02675 (542724) 542724..544130 + 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
KFU86_RS02680 (544375) 544375..545520 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
KFU86_RS02685 (545538) 545538..546551 + 1014 WP_000220399.1 ABC transporter ATP-binding protein -
KFU86_RS02690 (546552) 546552..547493 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T200985 WP_000813794.1 NZ_CP073996:542007-542183 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T200985 NZ_CP098206:230435-230542 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAGCTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT200985 WP_001270286.1 NZ_CP073996:542229-542645 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT200985 NZ_CP098206:c230378-230321 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References