Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 76322..76965 Replicon plasmid pYLMB1
Accession NZ_CP073995
Organism Escherichia coli strain MB1

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag KFU72_RS23525 Protein ID WP_001034044.1
Coordinates 76549..76965 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag KFU72_RS23520 Protein ID WP_001261286.1
Coordinates 76322..76552 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU72_RS23500 (72953) 72953..73708 - 756 WP_000852146.1 replication initiation protein RepE -
KFU72_RS23505 (74430) 74430..75236 - 807 WP_000016970.1 site-specific integrase -
KFU72_RS23510 (75237) 75237..75542 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
KFU72_RS23515 (75544) 75544..75762 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
KFU72_RS23520 (76322) 76322..76552 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KFU72_RS23525 (76549) 76549..76965 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
KFU72_RS23530 (77040) 77040..78605 + 1566 WP_001128474.1 AAA family ATPase -
KFU72_RS23535 (78590) 78590..79612 + 1023 WP_000361402.1 helicase UvrD -
KFU72_RS23540 (79866) 79866..80552 - 687 Protein_98 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul1 / qacE / aadA5 / blaCTX-M-15 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / mph(A) / sitABCD iutA / iucD / iucC / iucB / iucA 1..98811 98811


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T200984 WP_001034044.1 NZ_CP073995:76549-76965 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T200984 NZ_CP098205:39900-40154 [Escherichia coli]
ATGAGAAAAAATAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT200984 WP_001261286.1 NZ_CP073995:76322-76552 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT200984 NZ_CP098205:40151-40393 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References