Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4289902..4290399 Replicon chromosome
Accession NZ_CP073994
Organism Escherichia coli strain MB1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KFU72_RS20770 Protein ID WP_001173343.1
Coordinates 4289902..4290075 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag KFU72_RS20775 Protein ID WP_001425728.1
Coordinates 4290103..4290399 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU72_RS20760 (4286572) 4286572..4288029 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
KFU72_RS20765 (4288266) 4288266..4289783 + 1518 WP_001295074.1 lysine--tRNA ligase -
KFU72_RS20770 (4289902) 4289902..4290075 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KFU72_RS20775 (4290103) 4290103..4290399 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KFU72_RS20780 (4290626) 4290626..4290898 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
KFU72_RS20785 (4290910) 4290910..4291140 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
KFU72_RS20790 (4291321) 4291321..4292952 + 1632 WP_001216487.1 sensor histidine kinase -
KFU72_RS20795 (4292949) 4292949..4293668 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - papD / papC 4219662..4290399 70737


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T200978 WP_001173343.1 NZ_CP073994:c4290075-4289902 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T200978 NZ_CP098203:c3837451-3836531 [Escherichia coli]
ATGGATCTGCGTGATAGCTATGCCAGGCAGGTTAAACTGTTGATGGCCGCTCTGCCGCATGTGGCGAAGGAATCCTGTTT
CGCGTTGAAAGGCGGCACAGCAATCAATTTATTTGTGCAGGATTTCCCCCGTCTATCGGTGGATATCGATCTGGCGTATA
AGAGCTTTGCGGATCGCGATACCGATCTGGCGGCGATCAACGACGCTTTGAAGAGAATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATATGTGCTGCGCTTGATCGTCAGCAT
CCGCGCGATCTGTTTGATGTGTTGAATATGCTGGGGAGACCGGGCCTGACGCGCGAAATTTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGTCCCATTGCCGAGTTGCTGGCTTCAAACTGGGATACAGATCGGATTGCCACGTTATACGCTC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTTCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATTTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGCAATCTGGCGATGCTTAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGGAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT200978 WP_001425728.1 NZ_CP073994:c4290399-4290103 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT200978 NZ_CP098203:c3838220-3837438 [Escherichia coli]
TTGCCAAACTATCTAAACTGGTTGATGCAAAACACGCTACCCGGTCAGGTGCTGCTCCAGTCATGGCTTACCCGCAATGG
CGTAGATCGTAAGCTTTCTTATCTCTATGTTCAGAATGGCTGGCTCAGGCGCATCGCTCACGGTGTGTACTGCCGGACGG
GCCGTGAGCCGGATTGGGTAGACGCGGTTTACTGCCTTCAAACACAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGCCATGCGCACTATACCGAACCGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTTGCTGCCTTCGAACAATCAGCAACATTTATCACGCTGTATACATCGGGCCTTACCGTTGATGTCT
CGTCATTTACTACGGAACTGAAAATTGGCGACCGGCAGTTAGCAGGCAGTGCACCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTGATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGGGGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGTCATTCGATTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATCTTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCTGGTGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCCCGCACATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References