Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3493533..3494151 Replicon chromosome
Accession NZ_CP073994
Organism Escherichia coli strain MB1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KFU72_RS16930 Protein ID WP_001291435.1
Coordinates 3493933..3494151 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KFU72_RS16925 Protein ID WP_000344800.1
Coordinates 3493533..3493907 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU72_RS16915 (3488622) 3488622..3489815 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KFU72_RS16920 (3489838) 3489838..3492987 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KFU72_RS16925 (3493533) 3493533..3493907 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KFU72_RS16930 (3493933) 3493933..3494151 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KFU72_RS16935 (3494323) 3494323..3494874 + 552 WP_000102568.1 maltose O-acetyltransferase -
KFU72_RS16940 (3494990) 3494990..3495460 + 471 WP_000136192.1 YlaC family protein -
KFU72_RS16945 (3495624) 3495624..3497174 + 1551 WP_001372022.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KFU72_RS16950 (3497216) 3497216..3497569 - 354 WP_000878140.1 DUF1428 domain-containing protein -
KFU72_RS16960 (3497948) 3497948..3498259 + 312 WP_000409911.1 MGMT family protein -
KFU72_RS16965 (3498290) 3498290..3498862 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T200976 WP_001291435.1 NZ_CP073994:3493933-3494151 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T200976 NZ_CP098203:c3624688-3624386 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT200976 WP_000344800.1 NZ_CP073994:3493533-3493907 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT200976 NZ_CP098203:c3624350-3624009 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References