Detailed information of TA system TA200964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1330854..1331479
• Replicon: chromosome
• Accession: NZ_CP073994
• Organism: Escherichia coli strain MB1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KFU72_RS06445
• Protein ID: WP_000911330.1
• Coordinates: 1331081..1331479 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: KFU72_RS06440
• Protein ID: WP_000450524.1
• Coordinates: 1330854..1331081 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU72_RS06415 (1326657)	1326657..1327127	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
KFU72_RS06420 (1327127)	1327127..1327699	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
KFU72_RS06425 (1327845)	1327845..1328723	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
KFU72_RS06430 (1328740)	1328740..1329774	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
KFU72_RS06435 (1329987)	1329987..1330700	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
KFU72_RS06440 (1330854)	1330854..1331081	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
KFU72_RS06445 (1331081)	1331081..1331479	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
KFU72_RS06450 (1331626)	1331626..1332489	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
KFU72_RS06455 (1332504)	1332504..1334519	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
KFU72_RS06460 (1334593)	1334593..1335291	+	699	WP_000679823.1	esterase	-
KFU72_RS06465 (1335401)	1335401..1335601	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T200964 WP_000911330.1 NZ_CP073994:1331081-1331479 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T200964 NZ_CP098203:333185-333712 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT200964 WP_000450524.1 NZ_CP073994:1330854-1331081 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT200964 NZ_CP098203:332912-333178 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33