Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 889012..889666 Replicon chromosome
Accession NZ_CP073994
Organism Escherichia coli strain MB1

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KFU72_RS04345 Protein ID WP_000244777.1
Coordinates 889259..889666 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KFU72_RS04340 Protein ID WP_000354046.1
Coordinates 889012..889278 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU72_RS04315 (884300) 884300..885043 + 744 Protein_846 SDR family oxidoreductase -
KFU72_RS04320 (885100) 885100..886533 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
KFU72_RS04325 (886578) 886578..886889 + 312 WP_001679467.1 N(4)-acetylcytidine aminohydrolase -
KFU72_RS04330 (887053) 887053..887712 + 660 WP_000250274.1 hemolysin III family protein -
KFU72_RS04335 (887789) 887789..888769 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KFU72_RS04340 (889012) 889012..889278 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KFU72_RS04345 (889259) 889259..889666 + 408 WP_000244777.1 protein YgfX Toxin
KFU72_RS04350 (889706) 889706..890227 - 522 WP_001055874.1 flavodoxin FldB -
KFU72_RS04355 (890339) 890339..891235 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KFU72_RS04360 (891260) 891260..891970 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KFU72_RS04365 (891976) 891976..893709 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T200961 WP_000244777.1 NZ_CP073994:889259-889666 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T200961 NZ_CP098202:15255-15653 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGCATCAGTTCGGTCACTCTGATGGAGGTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGACGTTCTGGATTACGACGCTGCTGCTGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCTTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT200961 WP_000354046.1 NZ_CP073994:889012-889278 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT200961 NZ_CP098202:15028-15255 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCTGCCGGAGAGACATGGGACGAATGGTTCGACGGAA
ACAGTGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCTGGTATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References