Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 303103..303689 Replicon chromosome
Accession NZ_CP073994
Organism Escherichia coli strain MB1

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag KFU72_RS01370 Protein ID WP_042014162.1
Coordinates 303103..303333 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag KFU72_RS01375 Protein ID WP_000593555.1
Coordinates 303330..303689 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU72_RS01360 (299140) 299140..301875 + 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
KFU72_RS01365 (301875) 301875..302999 + 1125 WP_001314210.1 ABC transporter permease -
KFU72_RS01370 (303103) 303103..303333 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
KFU72_RS01375 (303330) 303330..303689 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KFU72_RS01380 (303809) 303809..304210 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
KFU72_RS01385 (304216) 304216..305022 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
KFU72_RS01390 (305019) 305019..305783 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
KFU72_RS01395 (305783) 305783..306616 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
KFU72_RS01400 (306613) 306613..307557 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T200957 WP_042014162.1 NZ_CP073994:303103-303333 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T200957 NZ_CP098201:c4902301-4902194 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT200957 WP_000593555.1 NZ_CP073994:303330-303689 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT200957 NZ_CP098201:4902349-4902415 [Escherichia coli]
GGTCTAGATGCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References