Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2228966..2229492 Replicon chromosome
Accession NZ_CP073988
Organism Escherichia coli strain MB10

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag KFU65_RS10915 Protein ID WP_000323025.1
Coordinates 2229205..2229492 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag KFU65_RS10910 Protein ID WP_000534858.1
Coordinates 2228966..2229205 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU65_RS10880 (2224302) 2224302..2224791 + 490 Protein_2124 class I SAM-dependent methyltransferase -
KFU65_RS10885 (2225269) 2225269..2225689 + 421 Protein_2125 hypothetical protein -
KFU65_RS10890 (2226345) 2226345..2227394 + 1050 Protein_2126 ISNCY family transposase -
KFU65_RS10895 (2227515) 2227515..2227664 + 150 WP_011443592.1 protein YdfW -
KFU65_RS10900 (2228101) 2228101..2228433 - 333 WP_001301033.1 FlxA-like family protein -
KFU65_RS10905 (2228636) 2228636..2228941 - 306 WP_001326990.1 protein YdfV -
KFU65_RS10910 (2228966) 2228966..2229205 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
KFU65_RS10915 (2229205) 2229205..2229492 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
KFU65_RS10920 (2229564) 2229564..2229719 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
KFU65_RS10925 (2229936) 2229936..2230187 + 252 WP_000980994.1 protein Rem -
KFU65_RS10930 (2230254) 2230254..2230532 + 279 WP_012304870.1 hypothetical protein -
KFU65_RS10935 (2230534) 2230534..2231583 + 1050 WP_001265198.1 DUF968 domain-containing protein -
KFU65_RS10940 (2231597) 2231597..2232349 + 753 WP_001047135.1 antitermination protein -
KFU65_RS10945 (2232627) 2232627..2232716 - 90 WP_122987542.1 hypothetical protein -
KFU65_RS10950 (2232771) 2232771..2232982 - 212 Protein_2138 cold shock-like protein CspF -
KFU65_RS10955 (2233281) 2233281..2233496 + 216 WP_000066485.1 cold shock-like protein CspB -
KFU65_RS10960 (2233860) 2233860..2234030 - 171 WP_001405948.1 putative zinc-binding protein YnfU -
KFU65_RS10965 (2234250) 2234250..2234465 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2211837..2265209 53372


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T200949 WP_000323025.1 NZ_CP073988:2229205-2229492 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T200949 NZ_CP098201:c2576321-2574999 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAATGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAACCGTTATGCCAGACCGTTATCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
TGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTGCCGGACGCAGAAATCATTAAAGCGGGGAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTGTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTATTGGGTGGCGCAGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGGTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTTCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGGCCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT200949 WP_000534858.1 NZ_CP073988:2228966-2229205 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT200949 NZ_CP098201:c2576587-2576321 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGTCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TGACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References