Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 821791..822445 Replicon chromosome
Accession NZ_CP073988
Organism Escherichia coli strain MB10

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KFU65_RS04035 Protein ID WP_000244777.1
Coordinates 822038..822445 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KFU65_RS04030 Protein ID WP_000354046.1
Coordinates 821791..822057 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU65_RS04005 (816960) 816960..817703 + 744 WP_000951948.1 SDR family oxidoreductase -
KFU65_RS04010 (817760) 817760..819193 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
KFU65_RS04015 (819238) 819238..819549 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
KFU65_RS04020 (819713) 819713..820372 + 660 WP_077150789.1 hemolysin III family protein -
KFU65_RS04025 (820568) 820568..821548 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KFU65_RS04030 (821791) 821791..822057 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KFU65_RS04035 (822038) 822038..822445 + 408 WP_000244777.1 protein YgfX Toxin
KFU65_RS04040 (822485) 822485..823006 - 522 WP_001055874.1 flavodoxin FldB -
KFU65_RS04045 (823118) 823118..824014 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KFU65_RS04050 (824039) 824039..824749 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KFU65_RS04055 (824755) 824755..826488 + 1734 WP_000813208.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T200939 WP_000244777.1 NZ_CP073988:822038-822445 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T200939 NZ_CP098201:585994-586284 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT200939 WP_000354046.1 NZ_CP073988:821791-822057 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT200939 NZ_CP098201:585762-586004 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References