Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 72097..72698 Replicon plasmid pYLMB15b
Accession NZ_CP073986
Organism Escherichia coli strain MB15

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag KFU92_RS25000 Protein ID WP_001216045.1
Coordinates 72097..72477 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag KFU92_RS25005 Protein ID WP_001190712.1
Coordinates 72477..72698 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU92_RS24960 (KFU92_24795) 67226..68077 + 852 WP_000611664.1 phage repressor protein C1 -
KFU92_RS24965 68110..68442 - 333 WP_224205061.1 phage antirepressor KilAC domain-containing protein -
KFU92_RS24970 68563..68913 - 351 Protein_65 ORF6N domain-containing protein -
KFU92_RS24975 (KFU92_24805) 69222..69443 - 222 WP_001361625.1 host cell division inhibitor Icd-like protein -
KFU92_RS24980 (KFU92_24810) 69462..69581 - 120 WP_071527722.1 ash family protein -
KFU92_RS24985 (KFU92_24815) 69750..70718 + 969 WP_000654811.1 IS5 family transposase -
KFU92_RS24990 (KFU92_24820) 70842..71885 - 1044 WP_072647226.1 DUF968 domain-containing protein -
KFU92_RS24995 (KFU92_24825) 71913..72092 - 180 WP_000113018.1 hypothetical protein -
KFU92_RS25000 (KFU92_24830) 72097..72477 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KFU92_RS25005 (KFU92_24835) 72477..72698 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KFU92_RS25010 (KFU92_24840) 72881..74437 + 1557 WP_072647227.1 type I restriction-modification system subunit M -
KFU92_RS25015 (KFU92_24845) 74434..75702 + 1269 WP_106490977.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..108307 108307
- flank IS/Tn - - 69750..70718 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T200935 WP_001216045.1 NZ_CP073986:c72477-72097 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T200935 NZ_CP098200:19662-19949 [Escherichia coli]
ATGACTTATACGGTTAAATTCAGGGATGATGCCCTCAGGGAATGGCTCAGACTGGATAAGGCCATTCAGCAGCAGTTCGC
AAAAAAACTAAAAAAATGCAGTGAAAATCCTCATATAGCCTCTGCAAAACTACGTGGATTAAAAGATTGCTACAAAATAA
AACTTCGCGCATCAGGTTTTCGTCTGGTCTATCAGGTGATTGATGACATGTTAATTATTGCGGTTGTCGCAGTAGGTAAA
CGTGAACGTAGTCATGTTTACAATCTCGCCAGCGAGAGAATGAGATAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT200935 WP_001190712.1 NZ_CP073986:c72698-72477 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT200935 NZ_CP098200:19414-19665 [Escherichia coli]
ATGCCCAGTATTATTTTGTCTGACACCAGTGCAAGCGTCAGTGAACTGAAAAAAAATCCAATGGCGACAGTCAGTGCCGG
AGAGGGTTATCCTGTTGCCATTCTTAACCGGAATCAGCCTGCATTCTACTGTGTTCCAGCTGAGCTCTATGAAAGGATGC
TTGATGCCCTGGATGACCAGGAGCTGGTGAAACTGGTTACTGAGCGCAGCAATCAGCCACTACATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References