Detailed information of TA system TA200933

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 84083..85706
• Replicon: plasmid pYLMB15a
• Accession: NZ_CP073985
• Organism: Escherichia coli strain MB15

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: H9TH67
• Locus tag: KFU92_RS23800
• Protein ID: WP_000088046.1
• Coordinates: 84381..85706 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5Z6V2D1
• Locus tag: KFU92_RS23795
• Protein ID: WP_024136327.1
• Coordinates: 84083..84376 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU92_RS23765 (KFU92_23620)	79955..81196	+	1242	WP_001053338.1	VWA domain-containing protein	-
KFU92_RS23770	81287..81418	+	132	WP_000374059.1	hypothetical protein	-
KFU92_RS23775 (KFU92_23625)	81759..82040	-	282	WP_000077926.1	hypothetical protein	-
KFU92_RS23780 (KFU92_23630)	82090..82281	-	192	WP_000398480.1	hypothetical protein	-
KFU92_RS23785 (KFU92_23635)	82373..82714	-	342	WP_001151575.1	hypothetical protein	-
KFU92_RS23790 (KFU92_23640)	83087..83479	+	393	WP_000341066.1	hypothetical protein	-
KFU92_RS23795 (KFU92_23645)	84083..84376	+	294	WP_024136327.1	helix-turn-helix transcriptional regulator	Antitoxin
KFU92_RS23800 (KFU92_23650)	84381..85706	+	1326	WP_000088046.1	type II toxin-antitoxin system HipA family toxin	Toxin
KFU92_RS23805 (KFU92_23655)	85767..85973	+	207	WP_000134171.1	hypothetical protein	-
KFU92_RS23810	86075..86212	+	138	WP_233762388.1	hypothetical protein	-
KFU92_RS23815	86224..86466	+	243	WP_233762385.1	hypothetical protein	-
KFU92_RS23820 (KFU92_23665)	86479..87294	+	816	WP_001572439.1	HNH endonuclease signature motif containing protein	-
KFU92_RS23825 (KFU92_23670)	87548..87973	+	426	WP_001043843.1	hypothetical protein	-
KFU92_RS23830 (KFU92_23675)	88427..88664	+	238	Protein_86	type I restriction-modification system subunit M N-terminal domain-containing protein	-
KFU92_RS23835 (KFU92_23680)	88719..89018	+	300	WP_001572440.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / tet(A) / blaCTX-M-32	-	1..247187	247187

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (9-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50249.20 Da
• Isoelectric Point: 6.1927

>T200933 WP_000088046.1 NZ_CP073985:84381-85706 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T200933 NZ_CP098198:c35857-35705 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10946.35 Da
• Isoelectric Point: 10.0925

>AT200933 WP_024136327.1 NZ_CP073985:84083-84376 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Nucleotide

• Length: 294 bp

>AT200933 NZ_CP098198:35912-35969 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A3T0VBU1

Antitoxin

• AlphaFold DB: A0A5Z6V2D1